1st, rather than the kit's EDTA-that contains elution buffer, ten mM Tris, pH7-8 is Ordinarily used for the ultimate elution, particularly for samples which might be to generally be sequenced with PacBio chemistry, since even small quantities of EDTA can impede downstream processes included in sequencing library preparation. Nuclease-free water https://www.facebook.com/NewTechFind/posts/pfbid02FCJT8WjvgB8mNgNjQTCGa79StJCpF6BAoHDqcZkpHnEqaVkXqXfgRZQTWycL4Whel