The technologies of Genotyping by Sequencing (GBS) and Restriction site Associated DNA Sequencing (RAD-Seq) have emerged as dual catalysts in the domain of genetic variation detection, reshaping research paradigms from crop breeding to ecological evolution through their high-throughput and cost-effective strategies. This article provides a systematic analysis of the core principles un... https://www.cd-genomics.com/resource-gbs-rad-seq-principles-discrepancy-challenges.html